PDF | In the state of Sinaloa, Mexico, the yellow bean of Azufrado-Peruano, Nueva Granada race, which occupies most of the surface area to. Lao: Mac thoua frang heng, Mac thousa khiem. Mex: Alubia chica, frijol amarillo, azufrado, bayo gordo, blanco, canelo, cocona, garbancillo, mexicano, negro. Caracterización proteómica de granos de frijol azufrado (Phamseolus vulgaris By Magda Karina Camacho-Espinoza. About this book.

Author: Faekree Ninris
Country: Slovenia
Language: English (Spanish)
Genre: Photos
Published (Last): 24 December 2009
Pages: 114
PDF File Size: 9.95 Mb
ePub File Size: 16.32 Mb
ISBN: 637-5-65980-661-3
Downloads: 73627
Price: Free* [*Free Regsitration Required]
Uploader: Dizragore

Soil testing and plant analysis. High vit1 levels in seeds correspond to vacuole formation during embryo development. A novel plant ferritin subunit from soybean that is related to a mechanism in iron release. Ferritin extraction was done following the method of Lane and Skopp This is similar to what we found in the common bean, where no difference was observed in ferritin gene expression in any of the plant organs, neither among the different plant growth stages. Moreover, these authors report that vit1 over expression in yeast mutant ccc1 increased vacuolar iron in seeds three fold over control cells and increased manganese but not zinc or cadmium.

Structure and differential expression of the four members of the Arabidopsis thaliana ferritin gene family. Sampling, handling and analyzing plant organ samples. Variable amounts of translatable ferritin mRNA in bean leaves with various iron contents.

Research focusing on the analysis of the iron demand by the plant is abundant.

Frijol (chenek’) / Bean (chenek’)

Ferritin was quantified by spectrophotometer Jenway at a visible light wavelength of nm based on the following formula: In fact, mRNA’s for ferritin subunits cp2, cp3, cp5 were produced during leaf development in similar amounts from each gene.

Azufgado of photosystem I. However, from the nutritional point of view, the relationship between ferritin and iron content for breeding purposes has been less studied. Contrary to the similar transcriptional levels of the ferritin gene, there were differences in ferritin protein contents in the plant organs, cultivars and growth stages probably due to the fact that in different tissues protease leads to different amounts of ferritin.


Frijol Peruano (Azufrado-Higuera) Peruvian Bean

Analytical instruments for soils and plant analysis. Due to szufrado ability to sequester iron, ferritin possesses the dual function of detoxification and storage.

The increase in vacuolar iron content in seeds prove that vit1 gene plays an important role in iron homeostasis and its expression is not affected by iron availability, as opposed to other proteins involved in iron metabolism.

In this work mRNA levels remain constant, whereas protein content was variable along developmental stages and plant organs. Therefore, common bean iron fortification includes the analysis and understanding of the elements involved in its content and regulation including the ferritin gene and protein regulation for iron availability. Ferritin mRNA, protein and iron concentrations during soybean nodule development. Iron storage and ferritin in plants.

Contenido de hierro y expresión del gen ferritina en frijol común (Phaseolus vulgaris L.)

An introduction to statistical methods and data analysis. In fact, ferritin subunits may express differentially according to the species, metabolism, growth stage, or general iron homeostasis. Febrero de Aceptado: However no correlation was observed with iron content indicating an independent event between iron concentration and ferritin expression and protein azucrado. Iron storage in bacteria.

An rfijol analysis was conducted to determine iron content, ferritin gene activity azuvrado ferritin protein content, in different plant organs and growth stages of four common bean cultivars.

Van der Mark et al. There were significant differences among cultivars. Differences in iron content among plant organs are due to the fact that much of the iron in leaves, for example, is associated with the ferrodoxins required for photosynthesis as part of the reverse tricarboxylic acid cycle Fromme et al.


Besides, cp1 expression did not vary during plant growth in leaves and roots.

Frijol Peruano (Azufrado-Higuera) Peruvian Bean | Invertebral Fresh Produce

In our study we did not observe this since in the common bean, the ferritin gene expressed at similar levels in all plant organs and at all growth stages. Even though the levels of expression in leaf, stem, and seed were different among cultivars, the expression was stable across the growth cycle.

Certified standards Perkin Elmer were run with every determination Watson and Robert, ; Jones et al. Occurrence and expression of members of the ferritin gene zaufrado in cowpeas. In legumes, iron is located mostly in ferritin Ambe et al.

Ferritin is a class of iron storage and mineralization protein that plays a key role in the iron metabolism of most organisms Azufardo, ; Andrews et al.

A practical sampling, azurrado, analysis, and interpretation guide. Relative expression of ferritin gene. Primers and probe were designed from the 1 bp bean ferritin gene sequenceNCBIusing the software Primer Express version 2. One gram of ground lyophilized fresh organ was added with ammonium citrate 7.

Ferritin in bean leaves with constant and changing iron status.

Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1. Ferritin protein content was significantly higher in seeds There is also the possibility that the iron detected in bean organs is not only stored in the ferritin protein.